Zuogui pill has protective effect against oxidative stress injury in osteoblasts / 中国组织工程研究

ABSTRACT

BACKGROUND: Recent studies have found that oxidative stress plays an important role in osteoporosisOsteoporosis is closely related to aging, estrogen deficiency, nuclear factor E2 related factor 2, heme oxygenase 1 and reactive oxygen species production. OBJECTIVE: To explore the protective mechanism of Zuogui Pill on oxidative stress injury of osteoblasts. METHODS: The study protocol was approved by the Ethics Committee of the Second Affiliated Hospital of Harbin Medical University. Osteoblasts were isolated and cultured from the skull of neonatal Sprague-Dawley rats, and were divided into three groups Control group, model group and Zuogui Pill group. The oxidative stress injury model of osteoblasts was established with 300 μmol/L H2O2 in the model and Zuogui Pill groups. Osteoblasts in the Zuogui Pill group were cultured with Zuogui Pill containing serum, and the control group did not undertake any treatment. Levels of malondialdehyde and superoxide dismutase in osteoblasts were determined. The expression of nuclear factor E2 related factor 2 and heme oxygenase 1 protein in osteoblasts was detected by western blot. RESULTS AND CONCLUSION: Compared with the control group, the content of malondialdehyde increased significantly, and the level of superoxide dismutase decreased significantly in the model group. Compared with the model group, the content of malondialdehyde decreased significantly and the level of superoxide dismutase increased significantly in the Zuogui Pill group. Western blot results showed that nuclear factor E2 related factor 2 and heme oxygenase 1 expression in osteoblasts of the model group increased significantly compared with the control group, and the expression of nuclear factor E2 related factor 2 and heme oxygenase 1 in osteoblasts of Zuogui Pill group was significantly higher than that of the model group. To conclude, Zuogui Pill can protect osteoblasts from oxidative stress injury.