Toxic effects of different-concentration isoniazid on newborn rat osteoblasts in vitro / 中国组织工程研究

ABSTRACT

BACKGROUND: As a first-line antituberculosis drug, isoniazide exerts strong sterilization effect on the Tubercle bacillus inside and outside the cells. But the toxic effect of isoniazide on osteoblasts is never reported. OBJECTIVE: To investigate the effect of different concentrations of isoniazide on the proliferation and differentiation of osteoblasts from newborn Sprague-Dawley rats. METHODS: Isoniazide treatment at 0, 10, 20, 30, 40, 50, 60, and 100 mg/L was applied on passage 3 osteoblasts from newborn Sprague-Dawley rats. Then, osteoblast proliferation and differentiation was detected using cell counting kit-8 assay, alkaline phosphatase activity kit and immunohistochemical staining. RESULTS AND CONCLUSION: Compared with the control group, the proliferation of osteoblasts was significantly inhibited when the isoniazide concentration was 30 mg/L. In addition, increasing isoniazide concentrations inhibited alkaline phosphatase activity and type I collagen expression. To conclude, isoniazide at exorbitant concentration exerts adverse effects on the proliferation and differentiation of osteoblasts.