Association of Lipoprotein Lipase Gene Polymorphisms with Plasma Lipids in Ischemic Stroke Patients

ABSTRACT

BACKGROUND: Lipoprotein lipase (LPL) is a key enzyme in the processing of triglycerides and plays a central role in lipid metabolism. It has been reported that the polymorphisms in the LPL gene were associated with plasma concentra-tions of HDL cholesterol (HDL) and triglycerides (TG) in coronary heart disease. We evaluated the correlation between the LPL gene polymorphisms and blood lipids in ischemic stroke patients. METHODS: Ninety-six ischemic stroke patients and 88 controls were included in the study. We evaluated polymorphic sites in the LPL gene using the HindIII for the intron 8 and PvuII for the intron 6 to the polymerase chain reaction products in each group. Allele frequencies, polymorphism information contents (PIC), heterozygosity indices of HindIII and PvuII polymorphisms were calculated in each group. Correlations of total cholesterol, HDL cholesterol, TG, and LDL cholesterol levels in the serum with the HindIII and PvuII polymorphisms of the LPL gene were analyzed in each group. RESULTS: The H+ frequencies, 0.786 and 0.752, in the stroke and control groups respectively. The P+ frequencies were 0.623 and 0.710 in stroke and control groups respectively. No significant difference was observed in triglyceride, total cholesterol, HDL, and LDL. CONCLUSIONS: These findings suggest that the HindIII and PvuII polymorphisms in LPL gene may not be associated with the occurrence of ischemic stroke.