Reversal effect of solanine on multidrug-resistance of leukemia K562/ADR cells and its mechanism / 肿瘤
Tumor
;
(12): 1276-1281, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-848445
ABSTRACT
Objective:
To investigate the effect of solanine on multidrug resistance of leukemia K562/ADR cells, and to explore its mechanism.Methods:
K562/ADR cells were treated with different concentrations (5-40 μg/mL) of solanine alone or in combination with doxorubicin (DOX) for 24 h. The intracellular toxicity and DOX-sensitization effect of solanine were detected by CCK-8 assay. The cell-associated mean fluorescence intensity of DOX was detected by FCM method. The expressions of multidrug resistance-associated protein 1 (MRP1), c-Jun NH2-terminal kinase (JNK), and phosphorylated JNK (p-JNK) were determined by Western blotting.Results:
Solanine at non-toxic doses (5 and 10 μg/mL) significantly enhanced the cytotoxicity of DOX (both P < 0.05), and significantly increased the mean fluorescence intensity of DOX in K562/ADR cells in a dose-dependent manner (both P < 0.05). After treatment with 5 and 10 μg/mL solanine, the expression levels of MRP1 and p-JNK proteins were significantly decreased (all P < 0.05) in K562/ADR cells.Conclusion:
Solanine can reverse multidrug resistance of K562/ADR cells in vitro. The mechanism may be related to blocking JNK signaling pathway and downregulating MRP1 expression.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Tumor
Année:
2017
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS