The expression of CARD9 in breast cancer tissues and its clinical significance / 肿瘤

ABSTRACT

Objective: To investigate the expression of caspase recruitment domain containing protein 9 (CARD9) protein in breast cancer tissues and its clinical significance. Methods: The expressions of CARD9 mRNA and protein in breast cancer cells, normal breast cells, breast cancer tissues and adjacent normal tissues were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The relationship between CARD9 expression and clinicopathological features was analyzed. The expressions of nuclear factor-κB (NF-κB) signaling pathway-related factors p50, p65, interlukin-1β (IL-1β) and IL-12 mRNA in breast cancer tissues and adjacent normal tissues were detected by real-time fluorescent quantitative PCR. The expressions of p50, p65, inhibitor of NF-κB kinase α (IKKα) and phosphorylated-IKKα (p-IKKα) proteins in breast cancer tissues and adjacent normal tissues were detected by Western blotting. The proliferation of breast cancer cells with CARD9 over-expression was detected by WST-1 assay. Results: The expressions of CARD9 mRNA (P < 0.01) and protein (P = 0.012) in breast cancer tissues were higher than those in the adjacent normal tissues. The expressions of CARD9 mRNA in breast cancer T-47D cells (P = 0.021) and MCF-7 cells (P = 0.014) were higher than that in normal breast Hs578Bst cells. The expressions of CARD9 mRNA and protein in breast cancer ZR-75-1 cells were higher than those in normal breast Hs578Bst cells (P = 0.003 and P = 0.015). The expression of CARD9 protein was positively correlated with the tumor size and estrogen receptor (ER) (both P < 0.015). The expressions of p50 (P < 0.05) and p-IKKα (P < 0.01) in breast cancer tissues were higher than those in the adjacent normal tissues. The expressions of p50 (r = 0.622, P < 0.001), p65 (r = 0.392, P = 0.005), IL-1β (r = 0.685, P < 0.001) and IL-12 (r = 0.556, P < 0.001) mRNAs were positively correlated with the expression of CARD9 mRNA. Enhancing the expression of CARD9 could promote the proliferation of breast cancer cells (P < 0.05). Conclusion: CARD9 may promote the proliferation of breast cancer cells and is involved in the formation and development of breast cancer by activating the NF-κB signaling pathway.