Inhibitory effects of γ-tocotrienol on invasion and migration of human gastric cancer SGC-7901 cells and its mechanism / 肿瘤
Tumor
;
(12): 441-447, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-848577
ABSTRACT
Objective:
To investigate the effects of γ-tocotrienol (γ-T3) on the invasion and migration of human gastric cancer SGC-7901 cells as well as its possible molecular mechanism.Methods:
The different concentrations (0, 15, 30, 45, 60, and 100 μmol/L) of γ-T3 were used to treat SGC-7901 cells. Then the proliferation, migration and invasion of SGC-7901 cells were detected by CCK-8 assay, cell scratch wound healing assay and Transwell invasion assay, respectively. Furthermore, the expressions of cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF- κB) signal pathway proteins were detected by Western blotting.Results:
After treatment with different concentrations (15-100 μmol/L ) of γ-T3 for 24, 48 and 72 h, the proliferation of SGC-7901cells was evidently inhibited in a time- and dose-dependent manner (all P<0.01). After treatment with γ-T3 (15-60 μmol/L) alone or combined with tumor necrosis factor-alpha (TNF-α) (10 ng/mL) for 24 h, the migration and invasion abilities of SGC-7901 cells were significantly inhibited (all P<0.01). The expressions of NF-κB, NF-κB p65 and COX-2 proteins were significantly down-regulated in SGC-7901 cells after γ-T3 (15-60 μmol/L) treatment for 24 h (all P<0.01).Conclusion:
γ-T3 can inhibit the invasion and migration of human gastric cancer SGC-7901 cells. Its mechanism may be associated with blocking NF-κB signal pathway and reducing the expression of COX-2 protein.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Tumor
Année:
2017
Type:
Article
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