Ataxia-telangiectasia mutated gene-silencing inhibits the migration and invasion of human breast cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of ataxia-telangiectasia mutated (ATM) gene-silencing on the proliferation, migration and invasion of human triple-negative breast cancer MDA-MB-231 cells. Methods: The recombinant lentiviral vectors with ATM gene-targeted specific shRNA or the negative control sequence (as the negative control group) were infected into the human triple-negative breast cancer MDA-MB-231 cells to obtain the ATM gene-silencing cells. At the same time, the uninfected MDA-MB-231 cells was used as the blank control group. After screening by puromycin, the infection efficiency of each group was observed under a fluorescence microscope. The expressions of ATM mRNA and protein in MDA-MB-231 cells in the three groups were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The effects of ATM gene-silencing on proliferation, cycle distribution, migration and invasion of MDA-MB-231 cells were analyzed by MTT method, FCM, cell wound healing assay and Transwell assays, respectively. Results: The human triple-negative breast cancer MDA-MB-231 cells with stable ATM genesilencing were established successfully. Compared with the blank control and negative control groups, the proliferation and cell cycle distribution of MDA-MB-231 cells in ATM gene-silencing group had no significant change (all P > 0.05), but the migration and invasion abilities of MDA-MB-231 cells in ATM gene-silencing group were decreased significantly (all P<0.05). Conclusion: The down-regulation of ATM gene expression can significantly inhibit the migration and invasion of human triple-negative breast cancer MDA-MB-231 cells.