Inhibiting effect of menin protein on the growth of human gastric cancer cell line AGS through PI3K/Akt signal transduction pathway / 肿瘤

ABSTRACT

Objective: To investigate the effect of menin protein encoded by the multiple endocrine neoplasia type I (MEN 1) gene on proliferation of human gastric cancer AGS cells and its possible machanism. Methods: An adenovirus encoding MEN 1 gene was used to infect human gastric cancer AGS cells. The expression level of menin protein in AGS cells infected with recombinant adenovirus Ad-MEN1 was detected by Western blotting and immunofluorescence. The proliferation of AGS cells was tested by MTT method. The cell cycle distribution and apoptosis of AGS cells were analyzed by flow cytometry (FCM). The expressions of phospho-Akt (p-Akt) and nuclear factor-kappa B (NF-κB) which were related to phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB) signal pathway were detected by Western blotting. Results: The results of Western blotting and immunofluorescence showed that the expression of menin protein in gastric cancer cell line AGS was increased after Ad-MEN1 infection (P 0.05). The expressions of p-Akt and NF-κB p65 in AGS cells were decreased after the expression of menin in AGS cells was increased (P < 0.05). Conclusion: This study reveals that over-expression of menin can inhibit the growth of human gastric cancer cell line AGS. This machanism may be related to the inhibition of PI3K/Akt and NF-κB signal transduction pathways.