Study on the loss of heterozygosity of microsatellite DNA on chromosome 6 in human T-cell lymphoma / 肿瘤

ABSTRACT

Objective: To study the loss of heterozygosity (LOH) at 6 markers of microsatellite polymorphism on the chromosome 6 in T-cell lymphoma (TCL) to determine whether there existed tumor suppressor genes in the area related with the initiation and development of TCL. Methods: Six microsatellite polymorphism markers (D6S251, D6S275, D6S287, D6S267, D6S262, and D6S264) on the chromosome 6 were selected. We performed the amplification of microsatellite DNA with PCR, polyacrylamide gel electrophoresis and silver staining to detect LOH in 42 cases of T-cell lymphoma and the corresponding normal tissues. Results: LOH were detected at more than one locus in 13 out of 42 TCL patients (30.95%). Among the 6 loci, LOH occurred more frequently at D6S262 (10.3%), D6S287 (10.0%), and D6S267 (7.3%). No significant difference was found in LOH incidence between different clinicopathologic classifications (P > 0.05). Conclusion: The LOHs occur more frequently at markers D6S262, D6S287, and D6S267 on the chromosome 6q21 to 6q23. Cyclin C gene localized to chromosome 6q21 may be the candidate of tumor suppressor gene related with initiation and progression of TCL. Chromosome 6q21-6q23 may harbor a tumor suppressor locus which was related with TCL.