The expression and significance of hypermethylation of p16 and RASSF1A genes in cervical carcinoma tissues / 肿瘤

ABSTRACT

Objective: To study the expression and significance of aberrant methylation of p16 and Ras association domain family A (RASSF1A) in cervical carcinoma. Methods: The cervical samples were divided into three groups cervical carcinoma (CC) group (n = 40), cervical intraepithelial neoplasia (CIN) group (n =80), and normal control group (n=20). The aberrant methylation of p16 and RASSF1A were detected by using methylation-specific reverse transcriptase polymerase chain reaction (MSP). Results: Methylation of pl 6 and RASSF1A genes were not detected in normal control group. The positive rate of p16 gene methylation was significantly higher in CC group than that in CIN group (40.00% vs 12.5%). The difference was statistically significant (χ2 = 11.88, P < 0.05). The positive rate of RASSFIA gene methylation was significantly higher in CC group than that in CIN group (20.0 % vs 7.5%). There was significant difference (χ2 = 4.04, P < 0.05). The positive rates of RASSF1A and p16 genes methylation were significantly higher in CC group than CIN group (55.0% vs 17.5%). The difference was significant (χ2 = 17.12, P < 0.05). Conclusion: Methylation status in the promoter regions of p16 and RASSF1A genes correlated with biological behaviors of cervical carcinoma. They may provide help in assistant diagnosis and treatment of cervical carcinoma.