Effect of cordyceps sinensis mycelium on serum vasoactive intestinal peptide and substance P in mice with intestinal dysbacteriosis / 解放军医学杂志

ABSTRACT

Objective To observe the effect of Cordyceps sinensis mycelium on serum vasoactive intestinal peptide (VIP) and substance P (SP) in mice with dysbacteriosis induced by antibiotics. Methods Forty-eight healthy SPF BALB/c mice were randomly divided into the normal control group (normal drink), the dysbacteriosis model group (induced by oral administration of 0.5 g/L ceftriaxone sodium), the natural recovery group (oral sterile water to replace antibiotic after reproduction of dysbacteriosis), and Cordyceps sinensis mycelium treatment group (treated by intragastric administration of Cordyceps sinensis mycelium). The feces were collected without contamination, and the change in intestinal bacterial number was observed with the plate dilution method. The volatile fatty acid was detected by chromatography. The serum VIP and SP contents were assayed with enzyme linked immunosorbent assay (ELISA). Results Compared with the normal control group, the numbers of probiotics, volatile fatty acids and serum VIP significantly decreased in the model group, while the serum SP markedly increased (P<0.01). Compared with the natural recovery group, the bacteria number, the quantities of volatile fatty acids and serum VIP significantly increased after the Cordyceps sinensis mycelium treatment, while the serum SP significantly decreased (P<0.01, P<0.05). Conclusion Cordyceps sinensis mycelium may effectively adjust the proportion of the probiotics in the mice with dysbacteriosis, and the mechanism is apparently related to alteration in the VIP and SP.