Effect of HIP1 gene silencing on proliferation of PC-3 cells in human androgen-independent prostate tumor / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of Huntingtin-interacting protein1 (HIP1) gene silencing on the proliferation of PC-3 cells in a human androgen-independent prostate tumor. Methods pSlience-shHIP1, a shRNA expression vector targeting HIP1, was constructed and transfected into PC-3 cells by liposome. RT-PCR was adopted to detect the silencing effect of HIP1 gene. Western bloting was then used to confirm the effective target point. Moreover, the scratch assay and growth curve assay were performed to analyze the effect of HIP1 on the proliferation of PC-3 cells. Results The efficiency of HIP1 gene silencing was increased to 83% (P<0.01) after PC-3 cell transfection. Western blotting proved that this genetic fragment was the effective target point for the inhibition of HIP1. The scratch assay and growth curve assay showed that the proliferation of PC-3 cells was inhibited by silencing HIP1 gene. Conclusions The pSilence-HIP1 expression vector can specifically suppress the expression of the HIP1 gene. In addition, HIP1 gene silencing can inhibit the proliferation and migration of PC-3 cells.