Principal component analysis and discriminant analysis of 12 effective chemical constituents in Aurantii Fructus from different areas / 中草药

ABSTRACT

Objective: To analyze and compare the contents of 12 effective chemical constituents in Aurantii Fructus from different areas, and identify their source of origin by using principal component analysis and discriminant analysis. Methods: Using isonaringin, narigin, hesperidin, neohesperidin, meranzin hydrate, naringin, hesperidin, meranzin, marmin, nobiletin, tangeretin, and auraptene as index, HPLC method was used to determine the content, and the average percentage content was analyzed by principal component analysis and discriminant analysis. Results: Principal component analysis results showed that Aurantii Fructus from different areas had certain similarities, and the content of Aurantii Fructus from Jiangxi Province was relatively stable. Discriminant analysis results showed that the samples from Zhangshu, Yichun, Yuanjiang, Huaihua, Jinhua, and Lanxi could be distinguished, while the samples from Xin’gan in Jiangxi, Jinhua, and Lanxi in Zhejiang overlapped. Conclusion: This method is simple, convenient and reliable, which can be used to screen out the suitable areas for the growth of Aurantii Fructus, so as to lay a foundation for explaining the authenticity of Aurantii Fructus.