Identification of Fallopia multiflora by allele-specific polymerase chain reaction / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 951-956, 2019.
Article
Dans Chinois
| WPRIM
| ID: wpr-851345
ABSTRACT
Objective:
To establish a rapid molecular identification method for Fallopia multiflora and its adulterants.Methods:
Based on psbA-trnH sequences of F. multiflora and its adulterants, the SNP site was searched and the specific primers were designed. The allele-specific PCR amplification of F. multiflora and its adulterants from different producing areas was carried out and the reaction system was optimized.Results:
When the annealing temperature was raised to 48 ℃ with 30 cycle number, only the template DNA of F. multiflora could be amplified to obtain the specific 191 bp band whereas the diagnostic PCRs of the other adulterants were all negative.Conclusion:
It’s simple and reliable to identify the authenticity of F. multifloa by allele loci specific PCR.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2019
Type:
Article
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