Genetic diversity analysis of Gardenia jasminoides based on ISSR and SRAP molecular markers / 中草药

ABSTRACT

Objective To analyze of genetic diversity of Gardenia jasminoides and provide the information for the conservation and new variety breeding of G. jasminoides. Methods 12 ISSR primers and 9 primer combinations of SRAP were used to assess the polymorphisms, genetic diversity, and cluster analysis within 21 G. jasminoides materials from three populations. Results The results showed that 100 (80.00%) of 125 and 74 (92.50%) of 80 bands were polymorphic by ISSR and SRAP primers amplification, respectively. In ISSR results, the populations of species level of observed number of alleles (Na), effective number of alleles (Ne), Nei’s gene diversity (H), Shannon’s information index (I), total genetic diversity for species (Ht), and the mean heterozygosity with populations (Hs) were 1.461 3, 1.307 7, 0.173 1, 0.254 5, 0.239 1, and 0.173 1, respectively. Comparatively, for SRAP primers, the Na, Ne, H, I, Ht, and Hs value was 1.579 2, 1.342 1, 0.197 4, 0.295 9, 0.289 9, and 0.197 4, respectively. The coefficient of gene differentiation (Gst) for population was 0.276 2 and 0.318 9, which indicated that the within-population component accounted for 73.38% and 68.11%, respectively. The average mean of gene flow (Nm > 1) suggested that there certainly gene flow among the populations. UPGMA analysis showed that 21 samples were clustered into 2 branches, and a hierarchical dendrogram based on SRAP was more consistent with actual populations. Conclusion The gene diversity of G. jasminoides populations was high. The characteristics of genetic structure included genetic differentiation that occurs mainly within populations, which provided a reference for conservation and breeding of G. jasminoides germplasm.