Chemical constituents of Caulophyllum robustum and its antitumor activities / 中草药

ABSTRACT

Objective To study the chemical constituents of Caulophyllum robustum and its antitumor activities. Methods The chemical constituents were isolated and purified with silica gel column chromatography, dextran gel Sephadex LH-20, and other chromatographic methods. Their structures were determined by physicochemical and spectral analysis. Furthermore, the cytotoxicity of these chemical components against the NSCLC A549 cell line was measured by CCK-8 method. Results Eleven compounds were isolated and identified as collinsogenin (1), hederagenin (2), eechinocystic acid 3-O-α-L-arabinopyranoside (3), magnolamide (4), (-)-magnocurarine (5), columbamine (6), 4,4’-diphenylmethane-bis (methyl) carbamate (7), (-)-8,8’-dimethoxy-L-O-(β-D- glucopyranosyl) secoisolariciresinol (8), asechipuroside A (9), 2-[4-(3-hydroxy-1-propenyl)-2-methoxyphenoxy]-1,3-propanediol (10), and 1-linoleoylglycerol (11). Conclusion Compounds 1-2 and 4-11 are isolated from Caulophyllum robustum genus for the first time. Compounds 1 and 2 show moderate inhibited effect on the proliferation of NSCLC A549 cell line.