Diversity of endophytic bacteria in Astragali Radix analyzed by Illumina MiSeq high-throughput sequencing technology / 中草药

ABSTRACT

Objective This study aimed to analyze the diversity of endophytic bacterial communities, and to provide reference to the oriented processing technology, which helps to screen bacteria fermentation and conversion in the active ingredients of Astragali Radix. Methods The 16S rDNA V3-V4 region of Astragali Radix was sequenced by Illumina MiSeq high-throughput sequencing technology, and the abundance of species and other biological information were analyzed. Results The results showed that the numbers of effective sequences and OTUs for sample were 40 051 and 967, respectively. The number of sequencing was close to saturation, and the sequencing data volume was reasonable. The endophytic bacteria of Astragali Radix mainly belonged to Anderseniella, Bacillus, Burkholderia, Acinetobacter, Stenotrophomonas, and Oceanobacillus. Conclusion The diversity of endophytic bacteria was low in Astragali Radix. The dominant population of endophytic bacteria in Astragali Radix belongs to Anderseniella and Bacillus.