Optimization of extraction technology and stability of anthocyanins from Duchesnea indica by response surface methodology / 中草药

ABSTRACT

Objective To optimize extraction technology and evaluate the stability of anthocyanins from Duchesnea indica by response surface methodology. Methods Based on single factor experiment, the ethanol concentration, liquid-material ratio, extraction time, and extraction temperature were selected as independent variables, and the anthocyanin extraction ratio was selected as response value. The Box-Behnken design method and response surface methodology were used to optimize the extraction technology. Additionally, the effects of pH, temperature, organic acid, and oxidizer on the stability of anthocyanins were investigated. Results The optimized extraction technology ethanol concentration was 80%, liquid-material ratio was 101, extraction time was 93 min, and extraction temperature was 36 ℃. The ratio of the extraction of anthocyanins with the optimal technology was 1.126%. The stability experiments results showed that the anthocyanins should keep in acid and low temperature conditions. Adding citric acid and gallic acid enhanced its stability, but glycine and H2O2 decreased stability. Conclusion The extraction technology optimized by response surface methodology was scientific, reasonable, and feasible, and it can be applicable for the extraction of anthocyanins from D. indica. Moreover, the condition should be paid attention to in order to maintain the stability of anthocyanins from D. indica.