Cloning of AP1 Gene from Lonicera macranthoides "Xianglei" and analysis on its Bioinformatics and Space-time expression / 中草药

ABSTRACT

Objective: To clone the AP1 (Lm-XL-AP1) gene from a Special Variant Varieties of Lonicera macranthoides "Xianglei", and to analyze its bioinformatics and spatio-temporal expression. Methods: Amplifing the full length of Lm-XL-AP1 gene by RACE technique, using bioinformatics method to analyze homology and similarity of the gene, predicting the coding protein and analyzing the various physical and chemical properties. The expression of the gene in different parts of Lonicera macranthoides Special Variant Varieties was detected by fluorescence quantitative PCR (qRT-PCR). Results: The AP1 gene, containing a 729 bp ORF that encoding 242 amino acids, was cloned. And the similarity of the gene compared with the AP1 gene from the MADS-box gene family of Chrysanthemum lavandulifolium up to 80% (Containing a conserved sequence of MADS and K-box). Without transmembrane domain, AP1 was located in cell nucleus. It is expressed in various organs of Lonicera macranthoides Special Variant Varieties. Conclusion: For the first time, the AP1 gene which may be involved in the control of the expression of floral organ was cloned from the total RNA of Lonicera macranthoides Special Variant Varieties.