Study on HPLC fingerprint of Valeriana jatamansi / 中草药

ABSTRACT

Objective To study the fingerprint of Valeriana jatamansi by HPLC, which can be used for the evaluation of its quality control. Methods The Phenomenex Gemini C18 (250 mm × 4.6 mm, 5 μm) column was used with a mobile phase of methyl acetonitrile (A)-0.1% phosphoric acid (B) gradient elution, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, and the detection wavelength was 254 nm. Above all these would be used for determining the fingerprint. Results There were 17 common peaks were found in the fingerprint of V. jatamansi, within six peaks were identified. The similarity degrees of 15 batches of samples were more than 0.89. Three principal components were abstractly represented 17 components and evalusted. Conclusion The established method is simple, fast, reliable, and can be used for evaluating the quality of V. jatamansi.