Simultaneous determination of four constituents in Zizyphi Spinosae Semen by ASE-CAD / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2002-2006, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-852797
ABSTRACT
Objective:
To establish an accelerated solvent extraction (ASE)-charged aerosol detector (CAD) method for simultaneous detection of jujuboside A, jujuboside B, spinosin, and betulinic acid in Zizyphi Spinosae Semen.Methods:
The orthogonal design was applied to optimize the extraction parameters of the ASE system. The target compounds were detected by HPLC-CAD with the parameters as follow Thermo Syncronis C18 (100 mm × 3 mm, 3 μm) column, a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 0.5 mL/min, the column temperature was kept at 40℃. Detector was Corona Ultra CAD with 35℃ of nebulization temperature.Results:
Optimization of the ASE parameters with orthogonal design greatly improved the extraction efficiency; All the target compounds could be simultaneously determined in a single run. Good linear relationships (0.998 3-0.999 6) and high relative recoveries were 98.46%-102.02%.Conclusion:
The method is rapid, simple, accurate, and thus could be used for the quality control of Zizyphi Spinosae Semen.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2017
Type:
Article
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