Simultaneous determination of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin in Aronia melanocarpa berry by UPLC / 中草药

ABSTRACT

Objective: The ultra performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin in Aronia melanocarpa berry. Methods: Waters Acquity UPLC® HSS T3 C18 column (100 mm × 2.1 mm, 1.8 μm) was used. The mobile phase was methanol (A)-0.03% phosphoric acid in water (B) for the gradient elution with flow rate of 0.2 mL/min. The column temperature was 35℃. The determination wavelength for procyanidin B1, procyanidin B2 and procyanidin B4 was 280 nm, and that for rutin and quercetin was 360 nm. Results: There was a linear correlation between the concentration of procyanidin B1 2.2-44.0 μg/mL (r = 0.999 2), procyanidin B2 50-1 000 μg/mL (r = 0.999 5), procyanidin B4 3.6-54.0 μg/mL (r = 0.999 2), rutin 7.4-148.0 μg/mL (r = 0.999 4), and quercetin 2.1-42.0 μg/mL (r = 0.999 7). The average recovery rates of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin were 101.23% (RSD = 2.13%), 99.64% (RSD = 1.23%), 102.31% (RSD = 2.89%), 98.74% (RSD = 2.96%), and 103.53% (RSD = 2.64%), respectively. The average contents of procyanidin B1, procyanidin B2, procyanidin B4, rutin, and quercetin in Aronia melanocarpa berry were 129.78, 3 834.99, 196.02, 134.40 and 79.10 μg/g, respectively. Conclusion: The analysis method is simple, rapid, reproducible, accurate, and reliable, and can be used to identify and evaluate the quantitative determination of five constituents in A. melanocarpa berry.