Comparison on polysaccharide among five species of Angelicae Radix / 中草药

ABSTRACT

Objective: Comparing the quality among five species of Angelicae Radix (Danggui) samples, i.e. Chinese Danggui (CDG, the roots of Angelica sinensis), Japanese Danggui (JDG, the roots of A. acutiloba), Korean Danggui (KDG, the roots of A. gigas), Lovage root (LR, the roots of Levisticum officinale), and Angelica archangelica root (AAR, the roots of A. archangelica) by evaluating the contents of their bioactive components. Methods: The 3,5-dinitrosalicylic acid colorimetric method (DNS method) was developed to quantify the amount of polysaccharides in a total of 46 species of Angelicae Radix collected from different countries based on the optimization of sampling, comparing the differences of polysaccharide among various species of Angelicae Radix, etc. Results: The contents of polysaccharides were found to be significantly different among the five species of Angelicae Radix. The order from high to low amount was AAR (162.5 mg/g, n=9), LR (142.3 mg/g, n=4), JDG (126.8 mg/g, n=13), KDG (126.8 mg/g, n=13), and CDG (80.75 mg/g, n=12). For JDG sample, the contents of polysaccharide were significant variety among samples cultivated in Japan (131.37 mg/g, n=3), China (184.29 mg/g, n=4), and Korea (94.98 mg/g, n=4). Conclusion: The developed DNS method is suitable to accurately quantify the amount of polysaccharide in Angelicae Radix. The pharmaceutical efficacy is variety among the five Angelicae Radix resulting from the various contents of polysaccharide. These herbs can not be mixed or substituted in clinical use.