Optimization in purification technique of polypeptides from Periplaneta american with macroporous adsorption resin / 中草药

ABSTRACT

Objective: To explore the optimal separation of polypeptides from Periplaneta americana by selecting appropriate macroporous resins and optimizing purification technology. Methods: Taking the index component test and inhibition experiment of tumor cells in vitro method as indexes, macroporous resins type was chosen and single factor test was adopted to evaluate some factors affecting separating technology. The elution position was analyzed by Automatic amino acid analyzer. Results: Macroporous resin HP20 had the best separating efficiency and the best purification conditions were as follows: the content in P. americana liquid 0.3 g/mL equivalent to raw material, the volume of drug 2 BV, the adsorption rate 2 BV/h, the volume of 70% ethanol as eluant 2 BV with elution rate of 2 BV/h. The recovery rate of polypeptides of elution position could reach 88.53% and the bound amino acids (polypeptides) was 55.64%. Conclusion: This technology is reasonable and feasible and has good separation effect, which could be used for enrichment and purification of antineoplastic compounds from P. americana.