RAPD analysis of genetic diversity of Dioscorea opposita germplasm resources from Jiangxi and genetic stability of their plantlets / 中草药

ABSTRACT

Objective: To analyze the genetic diversity of 11 varieties of Dioscorea opposita germplasm resources from Jiangxi, and detect the genetic stability of the tissue cultured plantlets. Methods: RAPD-PCR was applied. Results: Twenty primers were screened out, 238 bands were amplified, 234 of them were polymorphic bands, the average amplified polymorphic bands were 11.7, and the ratio of polymorphic bands was 98.32%. The genetic similarity coefficient was 0.575 6-0.970 6 and the average similarity coefficient was 0.723 1. According to the results of UPGMA clustering analysis, the 11 D. opposita germplasm resources from Jiangxi province were divided into five categories The first class included D. bulbifera. The second class included D. opposita. The third class included D. opposita Nancheng 1, D. opposita, D. opposita, D. opposite, and D. opposita, which showed that D. opposita 1, D. opposita, D. opposita and D. opposite, had closer relationship with D. opposita came from Wenxian County, Jiaozuo City, Henan province. The fourth class included D. opposita, D. opposita Nancheng 2 and D. opposita. The fifth class included D. opposita. The RAPD analysis results of plantlets subcultured for six times by in vitro rapid propagation method of stem segment with a bud of D. opposita L. germplasm resources from Jiangxi province (treatment group) and their seedlings germinated from microtuber (control group) also showed that 11 yam varieties were spread out 126-179 identifiable bands and each variety appeared 5-18 mutation bands, which indicated plantlets subcultured for six times by in vitro rapid propagation method of stem segment with a bud of D. opposita. germplasm resources from Jiangxi province existed somaclonal variation, but their genetic similarity coefficient compared with their seedlings germinated from microtuber was still relatively high, illustrating that their genetic traits were still relatively stable and did not affect their genotype. Conclusion: The experimental results can provide the reliable basis for introduction breeding, resource improvement, variety identification, germplasm conservation, and their plantlet culture of D. opposita germplasm resources from Jiangxi province.