RAPD-PCR analysis on different germplasm resources of Curcumae Rhizoma / 中草药

ABSTRACT

Objective: To analyze the genetic diversity and genetic relationship in different cultivars of Curcumae Rhizoma (CR) and identified with molecular marker technique. Methods: Forty-two samples in four cultivars of CR germplasm resources were studied with RAPD-PCR marker. The genetic similar coefficient and genetic distance were analyzed by POPGEN32 software and clustered by UPGMA method. Results: Ten primers were selected from 90 random primers, a total of 83 loci were scored, among which 64 were polymorphic loci. The percentage of polymorphic loci was 77.5%. Genetic distance changed from 0.22 to 0.58. The dendrogram of different CR cultivars was clear. Conclusion: The abundant diversity of CR from different populations exists with significant genetic differentiation, which is the key for screening the germplasm resources of CR and the basis for breeding and biotechnological development of CR.