Simultaneous determination of ten nucleosides and bases contents in plants of Paris L. by HPLC / 中草药

ABSTRACT

Objective: In order to provide a theory basis for rational development and utilization of medicinal plants from Paris L., this study compared nucleosides (cytidine, uridine, inosine, guanosine, thymidine, adenosine and 2'-desoxyadenossine) and bases (including uracil, guanine, and adenine) contents in the different plants of Paris L.which collected from different places. Methods: RP-HPLC was applied in this study, Venusil MP C18(2) chromatographic column (250 mm × 4.6 mm, 5 μm) was used, the mobile phase was a methanol and water gradient, the flow rate was1.0 mL/min, the temperature of column was 35℃ and the UV detector was set at 260 nm. Sixteen batches of samples in Paris L. were measured by above method, and cluster analysis was used to analyze the results. Results: Ten nucleosides and bases from the plants of Paris L. were measured, a good linear relationship was showed (r2 > 0.999 1), and the average recovery was 96.04%-104.34%, RSD ≤ 2.94%. The samples in Paris L. from different places were divided into three categories by cluster analysis. All of the ten nucleosides and bases were detected in the 16 batches of samples; However, the mass fraction and component ratio of the ten substances were significantly different from each variety. The average of total nucleosides and bases content was ranged from 735.390 1 to 1 612.807 3 μg/g, and the order from high to low was: P. bashanensis > P. polyphylla var. pseudothibetica > P. delavayi var. petiolata > P. axialis > P. polyphylla var. stenophylla > P. polyphylla var. chinensis > P. polyphylla var. yunnanensis > P. marmorata > P. polyphylla var. minor > P. mairei. The mass fraction and component ratio of the ten substances also had a significant difference between the samples collected from different regions which were the same variety. Conclusion: This method is simple, accurate and reliable and has good reproducibility. It is appropriate for measuring the contents of the ten nucleosides and bases. Simultaneously, this study provids a scientific basis for an objective and complete knowledge of the material basis for the efficacy, and for an enrichment and development of evaluation indicators system of in the plants Paris L.