Study on neuroprotective active constituents from root and rhizome of Valeriana amurensis / 中草药

ABSTRACT

Objective: To screen the active constituents from the anti-AD active fraction from the roots and rhizomes of Valeriana amurensis and to elucidate the therapeutic basis for the neuroprotective effect of the roots and rhizomes in V. amurensis. Methods: The aective fraction of the roots and rhizomes in V. amurensis was separated with various chromatographic processes and the structures of the obtained compounds were identified by physicochemical analysis and various spectra data. The neuroprtective compounds on PC12 cells were screened by MTT assay. Results: Eleven compounds were isolated from the roots and rhizomes of V. amurensis, including five bisepoxy lignans of (+)-medioresinol-4,4'-di-O-β-D-glucopyranoside (1), (+)-syringaresinol-4,4'-di-O-β-D-glucopyranoside (2), prinsepiol-4-O-β-D-glucopyranoside (3), (+)-8,8'-dihydroxy-pinoresinol-4,4'-di-O-β-D-glucopyranoside (4), prinsepiol (5), and 6 iridoids of jatamanin A (6), 7-hydroxy-8-(hydroxymethyl)-4-methylenehexahydrocyclopenta[c] pyran-1(3H)-one (7), 4-hydroxymethyl-cyclopenta[c] pyran-7-carboxaldehyde (8), patriscabroside III (9), jatamanin E(10), and patrinoside (11). The Aβ1-42-induced PC12 cells injuries were alleviated by all the bisepoxy lignans with the concentration of 25, 12.5, and 5 μmol/L. Conclusion: Iridoids 6-10 are isolated from the roots and rhizomes of V. amurensis for the first time. Bisepoxy lignans are the therapeutic basis of neuroprotective effect in the roots and rhizomes of V. amurensis.