Genetic diversity of Chuanmingshen violaceum by SRAP markers / 中草药

ABSTRACT

Objective: In order to correctly identify the different germplasm resources of Chuanmingshen violaceum and gain the excellent germplasm resources, SRAP molecular marker was used to analyze the genetic diversity of C. violaceum. Methods: C. violaceum was collected from seven different areas, which included 24 samples, the DNA fingerprint of C. violaceum was constructed with SRAP molecular marker, and the genetic diversity was analyzed. Results: Totally 374 bands were amplified by 37 primer pairs, of which 283 bands were polymorphic, and the polymorphic percentage was 75.67%. The SRAP-based genetic similarity coefficient of all samples ranged from 0.7267 to 0.9239, with a mean of 0.8150. The analysis of molecular variance showed higher percentages of genetic variation within population. All the accessions could be distinguished by SRAP markers. The cluster analysis results showed that 63 accessions were classified into seven groups, which were correlated with the geographical distribution of the accessions to some degree. The accessions from Langzhong and Jintang had higher diversity. Conclusion: There actually exists plentiful genetic diversity among the genetic resources of C. violaceum. SRAP marker is a useful method for analyzing the genetic diversity among C. violaceum accessions.