Optimization of technique for add itional liquid fermentation of Poria cocos and comparison on chemical constituents from different sources of Poria / 中草药

ABSTRACT

Objective To optimize the technique for additional liquid fermentation of Poriacocos in fermentor and to compare the chemical constituents in natural Poria, fermented Poria, medicinal fermented Poria, and compound medicinal fermented Poria. Methods The additional liquid fermentations of Poriacocos in fermentor had been carried out among different initial fermentation media, such as nutrition medium, medicinal medium, and compound medicinal medium. An optimum initial fermentation medium had been screened and an optimum time of additional liquid fermentation had been determined. The chemical constituents fromthedifferent sources of Poria had been isolated and detected according to their physicochemical properties. Results The compound medicinal medium with Coicis Semen and Lycii Fructus was an optimum initial fermentation medium, and 120 h was an optimum time of additional liquid fermentation. The total saccharides, polysaccharides, amino acids, trace elements, and ashes in thedifferent sources of Poria had been detected. Conclusion There are considerable influences on liquid fermentation of Poriacocos in different initial fermentation media. There are rather big differences of chemical constituents from the different sources of Poria.