DNA fingerprint library construction and cluster analysis of Forsythia suspensa from different origins / 中草药

ABSTRACT

Objective: RAPD technique has been used to construct DNA fingerprints and analyze the genetic diversity in 14 batches of Forsythia suspensa from different origins. Methods: Primers with good polymorphism were screened from 45 RAPD primers combinations by PCR, and the DNA fingerprints for 14 batches of F. suspensa were constructed by RAPD technique. Results: Eleven RAPD primers with good polymorphism were screened from 45 RAPD primers by PCR amplification, and 80 bands were amplified by the 11 RAPD primers with average 7.27 bands for each primer, of which 67 were polymorphic and PPB was 83.8%. The genetic similarity (GS) ranged from 0.4875 to 0.9625 in 14 batches of F. suspensa from different origins, which showed they had rich genetic diversity. The result of cluster analysis showed that F. suspensa was correlated with sample origin and was consistent with species distribution. Conclusion: The DNA fingerprints for 14 batches of F. suspensa are established based on the RAPD amplified bands.