Study on DNA barcoding of medicinal plants in Chaenomeles Lindl / 中草药

ABSTRACT

Objective: To provide evidence for the molecular identification of medicinal plants in Chaenomeles Lindl. Methods: Nineteen ITS2 and psbA-trnH sequence samples of five species in Chaenomeles Lindl. were PCR amplified and sequenced. The intra-specific and inter-specific K-2P distances were calculated, and cluster analysis was performed using Neighbor-joining (NJ) method by MEGA 6.0. Results: The original plant of Chaenomeles Lindl. could be identified by the sequence differences of the ITS2 and psbA-trnH sequences. Different samples of Chaenomeles Lindl. were gathered together and could be distinguished by NJ tree. There were significant differences among the ITS2 secondary structures in five species of Chaenomeles Lindl. Conclusion: ITS2 and psbA-trnH are efficient barcodes for the authentication of plants of Chaenomeles Lindl.