Fingerprint and multi-components quantitative determination for Huoxuetongluo Injection by UPLC / 中草药

ABSTRACT

Objective: To establish a UPLC fingerprint of the compounds in Huoxuetongluo Injection, and to make a quantitative analysis. Methods: The Thermo C18 (100 mm × 3 mm, 1.7 μm) column was used with a mobile phase of methyl alcohol-0.05% formic acid gradient elution, the flow rate was 0.4 mL/min, the column temperature was 20℃, and the detection wavelength was 260 nm. Results: The fingerprint chromatography included 21 mutual peaks, of which 11 mutual peaks from Paeoniae Radix Rubra, 7 mutual peaks from Chuanxiong Rhizoma, and 3 mutual peaks from Persicae semen. The similarity among the batches was more than 0.98. Based on the retention time, and UV absorption spectra of reference compounds, five components, amygdalin, oxypaeoniflorin, albiflorin, paeoniflorin, and ferulic acid, were identified and quantified. Conclusion: The method is rapid, simple, and accurate, and can be used for the quality control of Huoxuetongluo Injection.