Protection of 20 Chinese materia medica extracts to Aβ25-35-induced SH-SY5Y cell injury using high content screening / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 267-274, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-853759
ABSTRACT
Objective:
To identify neuroprotective extracts with the protective effects on Aβ25-35-induced SH-SY5Y cell injury via high content screening (HCS).Methods:
Hoechst 33342/PI double staining method was used to screen neuroprotective extracts from 60 Chinese materia medica (CMM) extracts. Further more, the effects of neuroprotective extracts on Aβ25-35-induced changes in the levels of Caspase-3/7 were detected.Results:
The results showed that 17 extracts had obviously neuroprotective effects. Among the 17 extracts, 8 of them inhibited Aβ25-35-induced up-regulation of Caspase-3/7.Conclusion:
HCS is an efficient method to screen neuroprotective extracts with the protective effects of Aβ25-35-induced SH-SY5Y cell injury. The neuroprotective extracts have potential medicinal value in Alzheimer's disease.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Étude de dépistage
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2016
Type:
Article
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