Simultaneous determination of ten components in Xianglian Huazhi Pill by HPLC / 中草药

ABSTRACT

Objective:To develop an HPLC method for the simultaneous determination of paeoniflorin, liquiritin, magnolol, ferulic acid, hesperidin, costunolide, dehydrocostus lactone, synephrine, baicalin, and berberine in Xianglian Huazhi Pill. Methods: The chromatographic separation was achieved on a Waters Symmetry-C18 (150 mm × 4.6 mm, 3.5 μm) column with methanol- acetonitrile-waters (50∶45∶5, A) and methanol-0.1% phosphoric acid (4∶96, B) as mobile phases for gradient elution, at the flow rate of 1.0 mL/min; The column temperature was 45 ℃. Results: The results showed that the ten active components were well separated and showed good linearity, paeoniflorin (1.5-15.0 μg/mL), liquiritin (0.5-5.0 μg/mL), magnolol (1.0-10.0 μg/mL), ferulic acid (0.5-5.0 μg/mL), hesperidin (12.5-125.0 μg/mL), costunolide (2.5-25.0 μg/mL), dehydrocostus lactone (2.5-25.0 μg/mL), synephrine (0.75-7.5 μg/mL), baicalin (1.5-15.0 μg/mL), and berberine (0.75-7.5 μg/mL). The precision was good and RSD was less than 2.0%. The repeatability was good, and RSD was less than 2.0%. The stability was good in 12 h. The average recoveries were between 98.5%-101.4%, and RSD was less than 2.0%. Conclusion: The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of Xianglian Huazhi Pill.