Analysis on codon bias of farnesyl pyrophosphate synthase gene in Astragalus membranaceus / 中草药

ABSTRACT

Objective: Farnesyl pyrophosphate synthase (FPS) is the key enzyme in biosynthesis pathway of astragaloside IV. The purpose of the experiment was to provide the theory basis for selecting appropriate expression systems and regulating the content of astragaloside IV. Method: FPS gene coding sequence was cloned based on Astragalus membranaceus from Changbai Mountain. Synonymous codons usage of FPS gene was analyzed by EMBOSS and Codon W programs and compared with the genome of other seven plants, such as Zea mays and Artemisia apiacea, and E. coli. Results: FPS gene of A. membranaceus was bias toward the codon with A and T at the third codon position and there are 22 codons showing the significant differences between FPS gene of A. membranaceus and E. coli genome. Conclution: The codons need to be optimized to improve the expression level of FPS gene in E. coli.