Rapid identification on chemical constituents in fruits of Schisandra sphenanthera and Schisandra chinensis and discrimination markers by UPLC-Q-TOF-MSE combined with OPLS-DA / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2212-2218, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-854043
ABSTRACT
Objective:
To establish a method to fast identify the chemical constituents in the fruits of Schisandra sphenanthera and Schisandra chinensis by ultra performance liquid chromatography tandem with time-of-fight mass spectrometry with MSE data-acquistion mode (UFLC-Q-TOF-MSE) and provide the basis for high throughput characterization and distinction of the two two samples of Schisandra Michx by analyzing the remarkably different chemical components in the fruits between S. sphenanthera and S. chinensis.Methods:
To rapidly identify the main chemical constituents in the fruits between S. sphenanthera and S. chinensis by using time-dependent scan mode, and combine with orthogonal partial least squares discriminant analysis (OPLS-DA) method to acquire significantly different constituents in the two samples of Schisandra Michx.Results:
A total of 33 compounds were identified including 27 dibenzocyclooctene lignans. The differences of chemical compounds between the two sample groups could be obviously observed through the method of OPLS-DA in positive mode. There were 15 chemical ingredients of lignanes with significant differences identified in the two sample groups by comparison with retention time and mass spectra.Conclusion:
The present study provides the basis for rapidly qualitative analysis of constituents and quantity control of the fruits in S. sphenanthera and S. chinensis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Recherche qualitative
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2015
Type:
Article
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