Extraction technology for active constituents in Dracocephali Moldavici Herba and comparison on contents of Dracocephali Moldavici Herba from various habitats / 中草药

ABSTRACT

To study the extraction technology for the active constituents in Dracocephali Moldavici Herba (the aerial parts of Dracocephalum moldevica) and to compare their contents Dracocephali Moldavici Herba from various habitats. The contents of luteolin-7-O-glucuronide (I), apigenin-7-O-glucuronide (II), rosmarinic acid, diosmetin-7-O-glucuronide (III), tilianin, and acacetin-7-O-glucuronide (IV) in Dracocephali Moldavici Herba were measured using HPLC method. Based on single-factor test, and the influence of the extracting menstrua, menstruum dosage, and extracting time were investigated using orthogonal design method. Based on the optimal technology, the contents of I, II, rosmarinic acid, III, tilianin, and IV in Dracocephali Moldavici Herba from various habitats were compared. The optimal conditions for the extracting for one time, each time for 5 h with 50 times of amount of 40% ethanol. D. moldevica produced in Jimusar, Xinjiang had the higher contents of I, II, rosmarinic acid, III, tilianin, and acacetin-7-O-glucuronide. This extraction technology is reasonable, stable, and feasible. The contents of I, II, rosmarinic acid, III, tilianin, and IV in Dracocephali Moldavici Herba from different habitats have some differences. The multi-index contents of I, apigenin-7-O-glucuronide, rosmarinic acid, III, tilianin, and IV could reflect the quality of Dracocephali Moldavici Herba more comprehensively.