Extraction, isolation, and purification of polysaccharides from leaves of Acanthopanax gracilistylus and property of their moisture retention / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1167-1173, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-854226
ABSTRACT
Objective:
Polysaccharides from the leaves of Acanthopanax gracilistylus (AGSL) were extracted with smashing tissue extraction (STE), isolated and purified, and the properties of their moisture retention and moisture absorption were studied.Methods:
Extraction process for AGSL polysaccharides (AGSL-P) was optimized by using the spherical symmetrical design test, and then isolated and purified through alcohol precipitation, Sevage method, bleaching, dialysis, DEAE-Sepharose Fast Flow column, and Sephadex G-75 column, and the structure was identified by chemical experiment, IR, and NMR. The properties of the moisture retention and moisture absorption of AGSL-P, AGSL-P-1, and AGSL-P-2 were studied and compared with common humectants (polyethylene glycol 400 etc.) in relative humidity 43% and 75%.Results:
The optimal STE conditions for AGSL-P were as follows material-liquid ratio was 114.5, the extraction temperature was 71℃, and the extraction time was 257 s. With the best extraction conditions, the yield of AGSL-P was 1.62%. AGSL-P-1-1 was homogeneous polysaccharide with α-configuration, and it may contain glucose, rhamnose, and galactose. The results showed the absorbent capacity of AGSL-P-1 and AGSL-P-2 was superior to the common humectants, polyethylene glycol 400, and moisturizing ability of AGSL-P-2 was amount to polyethylene glycol 400.Conclusion:
A steady and convenient extraction technology for AGSL-P has been established using STE method, AGSL-P-1-1 homogeneous polysaccharide is isolated from the leaves of AGSL has been established using STE method, AGSL-P-1-1 is isolated from the leaves of AGSL for the first time, and AGSL-P-2 is an excellent moisturizer.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2015
Type:
Article
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