Comparison on contents of four active constituents between slab and branch barks of Eucommia ulmoides / 中草药

ABSTRACT

Objective: To determine the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucoside in the slab and branch barks of Eucommia ulmoides. Methods: The separation was performed on a Cosmosil C18 (250 mm ×4.6 mm, 5 μm) column with the gradient elution acetonitrile -0.1% phosphoric acid; The flow rate was 0.8 mL/min; The detection wavelength was 208 nm; The column temperature was at 25 ℃. Results: The average content of aucubin slab bark > branch bark; The average content of chlorogenic acid branch bark > slab bark; The average content of geniposide branch bark > slab bark; The average content of pinoresinol diglucosid slab bark > branch bark. In different origins, the average contents of the above four constituents are more certain differences. Conclusion: The method is simple, rapid, accurate, and there are significant differences in the contents of aucubin, chlorogenic acid, geniposide, and pinoresinol diglucosid from the different parts of E. ulmoides, which would provide the better technique support for the quality control of E. ulmoides.