Mechanism of Reduning Injection on anti-acute lung injury in rats based on cytokine storm / 中草药

ABSTRACT

Objective: To investigate the effect and mechanism of Reduning Injection on the rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS) from cytokine storm. Methods: The model of ALI was established by instilling LPS (6 mg/kg) to SD rats. The effects of iv administration of Reduning Injection with three doses (10.16, 5.08, and 2.54 g/kg) and control drug Dexamethasone (0.5 mg/kg) on IL-1β, TNF-α, IL-4, IL-6, IL-8, IL-10, ICAM-1, and NF-KB in lung homogenate and lung tissue pathology were observed. Results: Reduning Injection could reduce the levels of IL-1β, TNF-α, IL-4, IL-6, IL-8, IL-10, ICAM-1, and NF-KB in lung tissue of rats, and improve the morphologic changes of lung tissue. Conclusion: Reduning Injection could improve the pathological changes of lung tissue, and the important curative mechanism may be reducing the production and release of inflammatory mediators in ALI process induced by LPS.