Identification of donkey-hide and its counterfeits based on 1H-NMR metabolomics / 中草药

ABSTRACT

Objective: The aqueous extracts of donkey-hide and its counterfeits (horse-hide and mule-hide) were identified using 1H-NMR metabolomics to provide the basis for the identification and quality control of donkey-hide gelatin. Methods: The water-soluble components of the hairless donkey-hide and its counterfeits were extracted and the 1H-NMR technique was applied to analyzing the components. Moreover, the NMR data were analyzed and the component belongings were recognized using relevant software for the multivariate statistical analysis. Results: Forty-two water-soluble components were identified in donkey-hide by 1H-NMR. There were more acetate, leucine, valine, isoleucine and less lactate, serine, pyroglutamate, and creatine in the horse-hide than in the donkey-hide; While there were more acetate, creatine, alanine, choline and less glycerol and acetate in mule-hide than in donkey-hide. And they were all in a significant difference (P 1H-NMR metabolomics was established for the first time, which provides the experimental references for the donkey-hide identification and quality control study.