Simultaneous determination of seven components in Pingxiao Tables by UPLC-Q-TOF-MS / 中草药

ABSTRACT

Objective: To develop an UPLC-Q-TOF-MS method for the determination of seven components (naringin, hesperidin, strychnine, brucine, agrimol B, wogonin, and protocatechuic acid) in Pingxiao Tablets. Methods: The analysis was performed on a Waters Acquity BEH C18 column (100 mm×2.1 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as mobile phase, and the flow rate was 0.45 mL/min; MS scanning mode negativion; Sample volume 2.0 μL. Results: The linear relationship between the concentration and peak areas of the seven compounds were all linear (r>0.9996). The average recoveries (n=6) were between 99%-101%. Conclusion: This method is simple, accurate, and reliable to determine the seven contents in Pingxiao Tablets for the quality control.