Research on antiviral constituents in Re-Du-Ning Injection (I) / 中草药

ABSTRACT

Objective: To study the antiviral constituents from the active fraction of Re-Du-Ning (RDN) Injection. Methods: In this study, the active fraction of RDN Injection was screened by the mice model loaded with restraint stress infected with influenza virus. The investigation on this fraction led to the isolation and identification of compounds through various chromatographic techniques and spectroscopic methods. In addition, the in vitro activity on influenza virus A (A/PR/8/34 H1N1) of the flavonoids was evaluated in vitro by the method for the detection of anti-influenza virus neuraminidase activity. Results: Through the macroporous adsorption resin, 95% ethanol eluate of RDN Injection was proved to be the antivirus active fraction of RDN Injection. Twenty compounds were obtained and characterized as syringic acid (1), ferulic acid (2), benzoic acid (3), caffeic acid (4), p-hydroxy benzaldehyde (5), vanillin (6), 4-hydroxy-3-methoxy styrene acrylic acid (7), trans-p-hydroxy cinnamic acid (8), trans-o-hydroxy cinnamic acid (9), trans-cinnamic acid (10), 7-hydroxy-6-methoxy coumarin (11), 7-hydroxy-6, 8-dimethoxy coumarin (12), coumarin (13), 3-hydroxy-1, 2-bis (4-hydroxy-3-methoxyphenyl)-1-propanone (14), isorhamnetin (15), quercetin (16), luteolin (17), rutin (18), hyperoside (19), and luteolin-7-O-β-D-glucoside (20). Among them, luteolin exhibited the antivirus activity against Flu A virus. Conclusion: All the isolated compounds are reported from RDN Injection for the first time, and luteolin exhibits the most potential activity against H1N1.