Establishment of rapid propagation system of Magnolia officinalis subsp. biloba / 中草药

ABSTRACT

Objective: To establish a rapid propagation system of Magnolia officinalis subsp. biloba. Methods: Using peeling seeds of M. officinalis subsp. biloba as the initial explants, different media and various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results: The best culture medium of seeds germination was B5 + 6-BA 0.5 mg/L + NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS + 6-BA 2.5 mg/L + NAA 0.5 mg/L, and the propagation coefficient was 6.2; The best rooting medium was 1/2 MS + NAA 0.5 mg/L, and the rooting rate was over 84% after 30 d. Conclusion: Rapid propagation technique system is established in order to lay technique foundation for the industrial production of M. officinalis subsp. biloba.