Optimation of extraction technology for Xinning Effervescent Tablet by central composite design/response surface method / 中草药

ABSTRACT

Objective: To optimize the extraction technology for Xinning Effervescent Tablet (XNET) by central composite design/ response surface method. Methods: Based on single-factor test, the independent variables were ethanol volume fraction, extracting time, and solvent ratio, while the dependent variables were extracting transfer rates of paeoniflorin, hesperidin, and schisandrin, with their weight calculation for a composite score. Multiple linear regression and multivariate binomial fit were used to estimate mathematic relationship between independent variables and dependent variables. Response surface description was based on optimal mathematical model, and then the optimal technical conditions were chosen. Results: The best multi-binomial model simulation was as follows Y=-209.21 + 6.67 A + 39.68 B + 6.59 C + 0.077 AB-0.026 AC-0.59 BC-0.051 A2-8.37 B2-0.13 C2 (r=0.979 3, P < 0.01). Optimal extraction conditions were as follows ethanol refluxing extraction, ethanol volume fraction of 65%, extraction time of 2 h, and solvent ratio of 12 (mLg). Under these optimal conditions, the average extracting transfer rates of schisandrinthe, paeoniflorin, and hesperidin were 85.33%, 88.77%, and 93.18%, and the actual extracting rate of catalpol was 88.72%, matching with the predicted extraction rate of 89.22%. Conclusion: The established model can better reflect the relationship between the factors and indicators, with good and reliable predictability.