Comparison on content differences of four bioactive compounds in different Wujiwan decoction by HPCE internal standard method / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1573-1576, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-854539
ABSTRACT
Objective:
To study the content difference of berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, and paeoniflorin in Wujiwan traditional decoction, granule prescription, and compound granule using HPCE internal standard method.Methods:
Capillary zone electrophoresis (CZE) method was used for a complete separation of the components, which was achieved with 50 mmol/L borax buffer-methanol (21), constant voltage of 25 kV, pressure injection 5 kPa × 15 s, electrolyle sealing 5 kPa × 10 s, and column temperature of 15°C.Results:
The capillary electrophoresis method is simple and accurate. The contents of the three alkaloids in the granule prescription were obviously higher than those in traditional decoction and compound granule.Conclusion:
This method is instructive for clinical application and quality control of Wujiwan prescription and compound granule.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2014
Type:
Article
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