Effect of Actinidia chinensis polysaccharide on apoptosis of MFC and their orthotopic transplanted tumor of gastric cancer / 中草药

ABSTRACT

Objective: To explore the effect of Actinidia chinensis polysaccharide (ACP) on apoptosis of MFC cells and their orthotopic transplanted tumor of gastric cancer and the molecular mechanism. Methods: MTT method was used to detect the inhibitory rate of ACP on MFC cell proliferation and the expression of Mcl-1, Bcl-2, Bak, and Bcl-xl protein was detected by Western blotting; The model of orthotopic transplanted tumor of gastric cancer was established with OB anastomosis adhesive stick method; Cell apoptosis in tumor tissue was detected by TUNEL method; The expression of Bcl-2 and Bax was detected by immunohistochemistry method. Results: MTT results showed that with the increasing of ACP concentration, the inhibition of ACP on MFC cell proliferation became stronger and with the prolongation of time, the inhibition was increasing. Western blotting showed that ACP would downregulate the expression of Mcl-1, Bcl-2, and Bcl-xl protein and upregulate the expression of Bak protein when the MFC cells were treated for 24 h by ACP. The animal experiment showed that compared with the model group, the average positive indexes of ACP in mid- and high-dose ACP and 5-FU positive control groups in the detection of apoptosis by TUNEL were significantly increased (P < 0.01); Meanwhile, in all ACP groups the expression of Bcl-2 could be reduced and the expression of Bax was upregulated significantly (P < 0.01). Conclusion: ACP could induce apoptosis of gastric cancer cells, downregulate the expression of Mcl-1, Bcl-2, and Bcl-xl protein, and upregulate the expression of Bak and Bax protein, prompting that the antitumor mechanism of ACP is related with the apoptosis pathway in which the Bcl-2 family proteins are involved.