Your browser doesn't support javascript.
loading
Elk-3 Contributes to the Progression of Liver Fibrosis by Regulating the Epithelial-Mesenchymal Transition
Gut and Liver ; : 102-111, 2017.
Article Dans Anglais | WPRIM | ID: wpr-85470
ABSTRACT
BACKGROUND/

AIMS:

The role of Elk-3 in the epithelial-mesenchymal transition (EMT) during liver fibrogenesis remains unclear. Here, we determined the expression of Elk-3 in in vitro and in vivo models and in human liver fibrotic tissues. We also investigated the molecular relationships among Elk-3, early growth response-1 (Egr-1), and the mitogen activated protein kinases (MAPK) pathway during EMT in hepatocytes.

METHODS:

We established anin vitro EMT model in which normal mouse hepatocyte cell lines were treated with transforming growth factor (TGF)-β1 and a CCl4-induced liver fibrosis model. Characteristics of EMT were determined by evaluating the expression levels of related markers. The expression of Elk-3 and its target Egr-1 were analyzed using Western blotting. Gene silencing of Elk-3 was performed using an siRNA knockdown system.

RESULTS:

The expression levels of mesenchymal markers were increased during TGF-β1-induced EMT of hepatocytes. The expression levels of Elk-3 and Egr-1 were significantly (p<0.05) increased during the EMT of hepatocytes, in CCl₄-induced mouse liver fibrotic tissues, and in human liver cirrhotic tissues. Silencing of Elk-3 and inhibition of the Ras-Elk-3 pathway with an inhibitor suppressed the expression of EMT-related markers. Moreover, Elk-3 expression was regulated by p38 MAPK phosphorylation during EMT.

CONCLUSIONS:

Elk-3 contributes to the progression of liver fibrosis by modulating the EMT via the regulation of Egr-1 under MAPK signaling.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphorylation / Techniques in vitro / Facteurs de croissance transformants / Lignée cellulaire / Technique de Western / Mitogen-Activated Protein Kinases / Extinction de l&apos;expression des gènes / Hépatocytes / Petit ARN interférent / P38 Mitogen-Activated Protein Kinases Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Gut and Liver Année: 2017 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphorylation / Techniques in vitro / Facteurs de croissance transformants / Lignée cellulaire / Technique de Western / Mitogen-Activated Protein Kinases / Extinction de l&apos;expression des gènes / Hépatocytes / Petit ARN interférent / P38 Mitogen-Activated Protein Kinases Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Gut and Liver Année: 2017 Type: Article