Sterols from roots of Clerodendrum trichotomum and their anti-inflammatory activity / 中草药

ABSTRACT

Objective: To investigate the anti-inflammatory chemical constituents from the roots of Clerodendrum trichotomum. Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatographies, as well as prep-HPLC; Their structures were characterized on the basis of physicochemical properties and their spectral analysis; An in vitro anti-inflammatory method was applied to determine their activities against interleukin-8 (IL-8) production. Results: Four 24-ethylcholestane derivatives have been identified as 24-ethyl-7-oxocholesta-5, 22(E), 25-trien-3β-ol (1), decortinone (2), 22-dehydroclerosterol (3), and clerosterol (4). Conclusion: Coumpounds 1-3 are isolated from the roots of C. trichotomum for the first time. The absolute configuration of C-24 of compound 1 is determined to be S. In an in vitro anti-inflammatory assay, compound 1 is found to show a remarkable inhibition on IL-8 production induced by interleukin-1β (IL-1β) in HT-29 cells.